Microscopy
Leica Stellaris WLL confocal point laser scanning microscope
The Leica Stellaris WLL is an automated confocal laser scanning microscope allowing simultaneous acquisition of 4 fluorescent channels as well as a transmission bright field channel. The microscope has as excitation sources a 405 nm laser diode and a pulsed White Light Laser (WLL) ranging from 485 nm to 685 nm, adjustable in 1 nm steps. The usage of the new sensitive Power HyD S detectors offers improved detection efficiency and photon counting in the wavelength range of 410 nm to 850 nm.
Special features / modules:
- Measurement of lifetime-based information (FLIM = Fluorescent Lifetime Imaging Microcopy) via the TauSense technology provides additional insights from samples
- Large-area mosaic scans acquired via the LAS X Navigator and Navigator Expert module with the fully automated microscope stage
- Confocal super-resolution (LIGHTNING) scanning for enhanced imaging precision
- Transmitted Light Imaging which supports brightfield and Nomarski contrast (DIC), fully automated
- Advanced FRET and FRAP experiments
- Live-cell imaging for dynamic time-lapse and multi-position imaging
Following objectives are available:
| Postition | Objective | Numerical aperture |
| 1 | 10x, HCPL FLUOTAR, AIR | 0.3 |
| 2 | 20x, HC PL APO CORR CS2, Oil | 0.75 |
| 3 | 40x, HC PL APO motCORR CS2, Glycerine | 1.25 |
| 4 | 63x, HC PL APO CS2, Oil | 1.4 |
Unique feature of the microscope: Stellaris WLL (White Light Laser)
- 405 nm diode laser DMOD
- 485 – 685 nm pulsed White Light Laser (WLL)
- Laser wavelength and intensity are regulated by the integrated AOTF and AOBS
- Wavelength selection is adjustable with 1 nm precision
- Enables up to 8 independent parallel laser lines
Olympus FV1000 confocal laser scanning microscope
The Olympus FV1000 IX81 is a laser scanning confocal microscope with additional life cell imaging chamber. The imaging platform is operated using the software FluoView FV1000 2.0b.
The FV1000 is equipped with an environmental chamber which allows the user to maintain stable experimental conditions like 37°C, 90% humidity and 5% CO2 concentration for long-term life imaging if needed.
The FV1000 IX81 is equipped with 4 lasers:
| Laser | Wavelength (nm) | Output power |
| 1 | 458, 488, 515 | adjustable |
| 2 | 635 | adjustable |
| 3 | 559 | adjustable |
| 4 | 405 | adjustable |
All users are required to undergo a intensive training before using the microscope. Trainings are giving on inquiry by the core facility operator. A corresponding request form can be found in PPMS.