The BD FACS AriaIII cell sorter has an optically gel-coupled cuvette flow cell. The true fixed laser alignment system minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.

The FACS AriaIII can be operated with a 70, 85 or 100 µm nozzle depending on the sample type. Nozzles are readily accessible and easy to change. 

The cell sorter is equipped with 5 lasers:

Laser	Wavelength (nm)	Output power (mW)
1	488	20
2	561	50
3	633	18
4	405	50
5	near UV 375	7

The collection optics of the BD FACS AriaIII is set up to measure 18 color parameters + FSC/SSC. The standard configuration with band-pass and long-pass filters can be found in PPMS in form of a table under My Documents. Numerous swappable filters are available and can easily be installed.

The FACS AriaIII is able to sort 4 subpopulations at a time. Cells for sorting can be delivered in 12 x 75 mm, and 15 ml tubes. Cells can be sorted into 15 ml tubes (2-way sorting) or into 1.5 ml and 12 x 75 mm tubes (4-way sorting). The Automatic Cell Deposition Unit (ACDU) enables sorting onto slides and into plates (6-, 24-, 48-, 96- and 384-well plates). Sample input and collections tubes, plates, and slides can be temperature controlled to 4°C, 20°C, 37°C and 42°C respectively.

The BD FACS AriaIII is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting. Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment on the FACS AriaIII. A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment. 

 

Beckman Coulter (formerly Dako) MoFlo Legacy is a Jet-in-Air high speed cell sorter. The modular platform offers flexibility regarding the configuration of the optical bench. 

MoFlo is routinely operated with a 100 µm nozzle at 20 psi. Also available is a 70  µm nozzle for "high speed sorting" and a 150  µm nozzle for large cells or particles.

The cell sorter is equipped with 5 lasers:

Laser	Wavelength (nm)	Output power (mW)
1	488	10 - 200
2	561	10 - 100
3	405 (co-linear with 640)	10 - 100
4	640 (co-linear with 405)	10 - 100

The MoFlo is capable to detect 8 color parameters + FSC/SSC. The standard filter configuration with band-pass and dichroic mirrows of MoFlo can be found in PPMS under My Documents. Numerous swappable filters are available and can easily be installed.

MoFlo is equipped with a 4-way sorting module which facilitates the sorting of up to 4 subpopulations. Cells for sorting can be delivered in 1.5 ml, 12 x 75 mm, 15 ml, and 50 ml tubes. Cells can be sorted into almost all saleable tubes (1.5 ml, 12 x 75 mm, 15 ml, and 50 ml tubes for 2-way sorting; 1.5 ml, 12 x 75 mm tubes for 4-way sorting). The robotic arm “Cyclone” enables sorting into multi-well plates (6-well to 384-well), in some cell culture vessels (Petri dishes 5 – 10 cm in diameter), chamber slides, and onto microscope slides.

MoFlo Legacy is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting. Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment on MoFlo.  A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment.