The BD FACS AriaIII cell sorter has an optically gel-coupled cuvette flow cell. The true fixed laser alignment system minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.

The FACS AriaIII can be operated with a 70, 85 or 100 µm nozzle depending on the sample type. Nozzles are readily accessible and easy to change. 

The cell sorter is equipped with 5 lasers:

Laser	Wavelength (nm)	Output power (mW)
1	488	20
2	561	50
3	633	18
4	405	50
5	near UV 375	7

The collection optics of the BD FACS AriaIII is set up to measure 18 color parameters + FSC/SSC. The standard configuration with band-pass and long-pass filters can be found as download here or in PPMS under "My Documents". Numerous swappable filters are available and can easily be installed.

Deliver your cells for sorting in 5ml FACS tubes or 15 ml tubes. The FACS AriaIII is able to sort 4 subpopulations at a time. Cells can be sorted into 15 ml tubes (2-way sorting) or into 1.5 ml and 5 ml FACS tubes (4-way sorting). The Automatic Cell Deposition Unit (ACDU) enables sorting onto slides and into plates (6-, 24-, 48-, 96- and 384-well plates). Sample input and collections tubes, plates, and slides can be temperature controlled to 4°C, 20°C, 37°C and 42°C respectively.

The BD FACS AriaIII is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting. Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment on the FACS AriaIII. A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment. 

 

The FACS Discover S8 is a state-of-the-art spectral cell sorter. Combining spectral detection with real-time cell imaging (CellView Image Technology), the S8 empowers researchers to explore cellular complexity with unprecedented depth and clarity. The system featured BD CellView Image Technology enables the visualization and imaging of cells during sorting, providing valuable morphological and functional context alongside traditional fluorescence data.

The BD FACS Discover S8 is operated with three different nozzle sizes: 85µm, 100µm and 130µm nozzle.

With 5 lasers and 78 fluorescence APD detectors, the S8 supports high-parameter experiments, making it ideal for applications such as immune profiling, stem cell characterization and rare cell analysis. 

Laser	Wavelength (nm)	Output power (mW)
1 - UV	349	30
2 - Violet	405	50
3 - Blue	488	100
4 - Yellow-Green	561	50
5 - Red	637	100

The unique CellView Image Technology enables the detection of fluorophores which are excited by the blue 488nm laser.

Imaging (CellView) excitation wavelenght: 488nm.

Channel	Long Pass Filter	Emmision Filter	Typical fluorophore	DNA binding dye
1	LP505	534/46	GFP, YFP, FITC, AlexaFluor488, BB515, Spark Blue 515, Vio515, cFluor B515, RB 545, ...	Vybrant DyeCycle green, Sytox Green, NucSpot Live 488, SYTO 24, ...
2	LP570	600/60	RB 613, PE-CF594, PE-Dazzle594, PE-eFluor610, PE, ...	Sytox Orange, ...
3	LP675	788/225	RB 705, RB 780, RB 744, PerCP-Cy5.5, PerCP-eFluor710, PerCP-Fire780, PerCP-Vio700, PE-Cy5.5, PE-Cy7, PE-Fire 810, ...	Sytox AADvanced, Sytox Deep Red, DRAQ 5, ...

The samples for sorting should be delivered in 5ml FACS tubes. The BD FACS Discover S8 can sort up to 6 subpopulations simultaneously. Cells can be sorted into 50ml, 15ml and 5ml tubes and into 2ml and 1.5ml eppis. Automated plate sorting into 6-, 24-, 48-, 96- and 384-well plates is possible as well. The sorter consists of a temperature control unit, which enables the heating or cooling of samples and collections tubes to 4°C, 22°C, 37°C and 42°C.

The BD FACS Discovery S8 is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting. Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment. A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment.