Cell Sorters
BD FACS ARIA III
The BD FACS AriaIII cell sorter has an optically gel-coupled cuvette flow cell. The true fixed laser alignment system minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.
The FACS AriaIII can be operated with a 70, 85 or 100 µm nozzle depending on the sample type. Nozzles are readily accessible and easy to change.
The cell sorter is equipped with 5 lasers:
| Laser | Wavelength (nm) | Output power (mW) |
| 1 | 488 | 20 |
| 2 | 561 | 50 |
| 3 | 633 | 18 |
| 4 | 405 | 50 |
| 5 | near UV 375 | 7 |
The collection optics of the BD FACS AriaIII is set up to measure 18 color parameters + FSC/SSC. The standard configuration with band-pass and long-pass filters can be found as download here or in PPMS under "My Documents". Numerous swappable filters are available and can easily be installed.
Deliver your cells for sorting in 5ml FACS tubes or 15 ml tubes. The FACS AriaIII is able to sort 4 subpopulations at a time. Cells can be sorted into 15 ml tubes (2-way sorting) or into 1.5 ml and 5 ml FACS tubes (4-way sorting). The Automatic Cell Deposition Unit (ACDU) enables sorting onto slides and into plates (6-, 24-, 48-, 96- and 384-well plates). Sample input and collections tubes, plates, and slides can be temperature controlled to 4°C, 20°C, 37°C and 42°C respectively.
The BD FACS AriaIII is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting. Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment on the FACS AriaIII. A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment.
BD FACS Discover S8
The FACS Discover S8 is a state-of-the-art spectral cell sorter available soon at our core facility. Combining spectral detection with real-time cell imaging, the S8 empowers researchers to explore cellular complexity with unprecedented depth and clarity. This system features BD CellView Image Technology, enabling visualization and imaging of cells during sorting, providing valuable morphological and functional context alongside traditional fluorescence data. With five lasers and 78 fluorescence detectors, the S8 supports high-parameter experiments, making it ideal for applications such as immune profiling, stem cell characterization, and rare cell analysis. Whether you are studying cellular heterogeneity, developing novel panels, or sorting based on subtle phenotypic differences, the FACS Discover S8 offers a powerful and flexible solution to advance your research.