The Core Facility is equipped with two cell sorters, one research cell analyzer and a confocal laser scanning microscope.
BD FACSAriaIII Cell Sorter (Room 067.000 20 014.02-1)
The BD FACSAriaIII Cell Sorter has an optically gel-coupled cuvette flow cell. The true fixed laser alignment system minimizes startup time, improves experiment-to-experiment reproducibility, and enables automated daily quality control.
The FACSAriaIII can be operated with a 70, 85 or 100 µm nozzle depending on the sample type. Nozzles are readily accessible and easy to change.
The Cell Sorter is equipped with 5 lasers:
|Laser||Wavelength (nm)||Output power (mW)|
|5||near UV 375||7|
The collection optics of the BD FACSAriaIII is set up to measure 18 color parameters + FSC/SSC. The standard configuration with band-pass and long-pass filters can be found in PPMS in form of a table under My Documents. Numerous swappable filters are available and can easily be installed.
The FACSAriaIII is able to sort 4 subpopulations at a time. Cells for sorting can be delivered in 12 x 75 mm, and 15 ml tubes. Cells can be sorted into 15 ml tubes (2-way sorting) or into 1.5 ml and 12 x 75 mm tubes (4-way sorting). The Automatic Cell Deposition Unit (ACDU) enables sorting onto slides and into plates (6, 24, 48, 96, and 384-well plates). Sample input and collections tubes, plates, and slides can be temperature controlled to 4, 20, 37 and 42°C respectively.
The BD FACSAriaIII is exclusively operated by the CAnaCore staff. The intended use of the device is cell sorting! Registered users have to fill out a booking form in PPMS before they are able to make a sorting appointment on the FACSAriaIII. A "Cell Sorting Guide" which can be found in PPMS under My Documents provides the basic information on what to consider when setting up a sorting experiment.
Beckman Coulter MoFlo Legacy Jet-in-Air Cell Sorter (Room 067.000 20 014.02-1)
Research Cell Analyzer
BD LSRII Analyzer (Room 067.000 20 014.01)
Confocal Laser Scanning Microscope
Olympus FV1000 Confocal Microscopy System (Room 067.000 20 014.03)
FlowJo/SeqGeq Work Stations
CAnaCore has two powerful work stations for data analysis using either FlowJo or SeqGeq. Those work stations are bookable via PPMS and are of no charge.